F. Kierszenbaum et Mb. Sztein, Down-regulation of human B lymphocyte activities by a Trypanosoma cruzi membrane glycoprotein, INT J PARAS, 31(3), 2001, pp. 265-271
The effects of purified AGC10, a Trypanosoma cruzi membrane glycoprotein, o
n normal human B lymphocytes were studied in this work. In the presence of
AGC10, [H-3]-thymidine uptake by human peripheral blood mononuclear cells s
timulated with the B cell-specific mitogen SACI (killed Staphylococcus aure
us Cowan I) was markedly decreased. This alteration was accompanied by othe
rs such as decreased expression of the CD122 and CD132 chains of the IL-2R
complex. These inhibitory effects appeared to be somewhat selective, as exp
ression of CD25, another IL-2R chain, was not affected by AGC10 and no sign
ificant modification occurred in the expression of the B-cell-specific mark
er CD19 or CD21. In contrast, AGC10 did reduce the levels of expression of
CD86 and CD80, molecules known to play critical roles in B cell interaction
s with T lymphocytes. Fairly large subpopulations of, but not all, B lympho
cytes had their expression of CD122(+), CD132(+), CD86(+) and CD80(+) reduc
ed to undetectable levels in the presence of AGC10. However, the SACI-activ
ated B cells that remained capable of expressing these molecules in the pre
sence of AGC10 did so at normal levels. This was denoted by comparable mean
fluorescence intensity values representing the expression of CD122, CD132,
CD86 or CD80 molecules on the surface of SACI-stimulated CD19(+) cells cul
tured without or with AGC10. These results indicated that AGC10, derived fr
om an organism that causes immunosuppression in infected hosts, down-regula
tes B cell activities and suggested that the relevant mechanism could invol
ve the molecular alterations described above. (C) 2001 Australian Society f
or Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved
.