Saccharopolyspora flava sp nov and Saccharopolyspora thermophila sp nov., novel actinomycetes from soil

The generic position of two aerobic, Cram-positive, non-acid-alcohol-fast a ctinomycetes was established following the isolation of their PCR-amplified 16S rRNA genes and alignment of the resultant sequences with the correspon ding sequences from representatives of the families Actinosynnemataceae and Pseudonocardiaceae. The assignment of the organisms to the genus Saccharop olyspora was strongly supported by chemotaxonomic and morphological data. T he strains were distinguished both from one another and from representative s of validly described Saccharopolyspora species on the basis of a number o f phenotypic properties. It is proposed that the organisms, strains 07(T) ( = AS4.1520(T) = IFO 16345(T)= JCM 10665(T)) and 216(T)(= AS4.1511(T) = IFO 16346(T) = JCM 10664(T)), be classified in the genus Saccharopolyspora as S accharopolyspora flava sp, nov. and Saccharopolyspora thermophila sp, nov., respectively.