An Actinomyces-like bacterium was recovered from two dogs. Based on cellula
r morphology and biochemical criteria, the unknown bacterium resembled the
genus Actinomyces but it did not appear to correspond to any of the current
ly recognized species of this genus. PAGE analysis of whole-cell proteins c
onfirmed that the strain was phenotypically distinct from all other Actinom
yces species and comparative 16S rRNA gene sequencing showed that the bacte
rium represents an unknown sub-line within the genus. Based on phenotypic a
nd phylogenetic evidence, it is proposed that the bacterium from dogs be cl
assified as a new species of the genus Actinomyces, Actinomyces catuli. The
type strain of Actinomyces catuli is CCUG 41709(T) (= CIP 106507(T)).