I. Yumoto et al., Pseudomonas alcaliphila sp nov., a novel facultatively psychrophilic alkaliphile isolated from seawater, INT J SY EV, 51, 2001, pp. 349-355
Citations number
41
Categorie Soggetti
Microbiology
Journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Facultatively psychrophilic alkaliphilic strains were isolated from seawate
r obtained off the coast of Rumoi, Hokkaido. Japan. They were Gram-negative
, aerobic straight rods with polar flagella, The isolates were catalase- an
d oxidase-positive and able to grow at 4 degreesC, but not at 40 degreesC,
They produced acid from D-glucose under aerobic conditions. The isolates re
duced nitrate to nitrite and hydrolysed casein and gelatin, but not starch
or DNA, NaCl was required for growth at ph 10 but was not required at neutr
al ph, The major isoprenoid quinone was ubiquinone-9 (0-9) and the DNA G+C
content was 62.3-63.2 mol%, The whole-cell fatty acids mainly consisted of
C-16:0, C-16:1(9c) and C-18:1(9c), with 3-OH C-10:0 and 3-OH C-12:0 as the
hydroxyl fatty acids. A larger amount of trans-unsaturated fatty acid, C-16
:1(9t) was observed when the cells were grown at ph 7 compared to when cell
s were grown at pH 10. Phylogenetic analysis based on Dentistry,165 rRNA ge
ne sequencing indicated that the bacteria are members of the genus Pseudomo
nas. Analysis of DNA-DNA relatedness data with several close National Insti
tute of phylogenetic neighbours revealed a low level of hybridization (less
than 61%), On the basis of phenotypic characteristics, phylogenetic analys
is and DNA-DNA relatedness data, it is concluded that these isolates repres
ent a separate new Japan species. Accordingly, the name Pseudomonas alcalip
hila is proposed. The type strain is AL15-21(T)(= JCM 10630(T) = IAM 14884(
T)).