Evaluation of laser-Doppler perfusion imaging for measurement of blood flow in cortical bone

Citation
Rc. Shymkiw et al., Evaluation of laser-Doppler perfusion imaging for measurement of blood flow in cortical bone, J APP PHYSL, 90(4), 2001, pp. 1314-1318
Citations number
18
Categorie Soggetti
Physiology
Journal title
JOURNAL OF APPLIED PHYSIOLOGY
ISSN journal
87507587 → ACNP
Volume
90
Issue
4
Year of publication
2001
Pages
1314 - 1318
Database
ISI
SICI code
8750-7587(200104)90:4<1314:EOLPIF>2.0.ZU;2-9
Abstract
Most techniques currently available to measure blood flow in bone are time consuming and require destruction of the tissue, but laser-Doppler technolo gy offers a less invasive method. This study assessed the utility of laser- Doppler perfusion imaging (LDI) to measure perfusion in cortical bone. Twel ve mature New Zealand White rabbits were assigned to one of three groups: n ormal control, constriction (norepinephrine), or dilatation (nitroprusside) . The left and right medial tibiae were consecutively scanned at red (634-n m) and near-infrared (810-nm) wavelengths to examine the repeatability of L DI output. The pharmacological intervention groups were injected with the r espective drug, and LDI measurements at 810 nm were obtained concurrently w ith colored microsphere-determined flow in all of the groups. LDI effective ly quantified blood flow in cortical bone and detected physiologically indu ced changes in perfusion. A significant positive correlation was found betw een microsphere-determined flow and LDI output (r = 0.6, P < 0.05). Repeata bility of consecutive LDI measurements was within 5%. The effectiveness of LDI to measure perfusion in bone suggests this method has potential for inv estigating the role of blood flow in bone metabolism and remodeling.