Acute exercise and training increase insulin action in skeletal muscle, but
the mechanism responsible for this effect is unknown. Activation of the in
sulin receptor initiates signaling through both the phosphatidylinositol (P
I) 3-kinase and the mitogen-activated protein kinase [MAPK, also referred t
o as extracellular signal-regulated kinases (ERK1/2)] pathways. Acute exerc
ise has no effect on the PI3-kinase pathway signaling elements but does act
ivate the MAPK pathway, which may play a role in the adaptation of muscle t
o exercise. It is unknown whether training produces a chronic effect on bas
al activity or insulin response of the MAPK pathway. The present study was
undertaken to determine whether exercise training improves the activity of
the MAPK pathway or its response to insulin in obese Zucker rats, a well-ch
aracterized model of insulin resistance. To accomplish this, obese Zucker r
ats were studied by using the hindlimb perfusion method with or without 7 w
k of treadmill training. Activation of the MAPK pathway was determined in g
astrocnemius muscles exposed in situ to insulin. Compared with lean Zucker
rats, untrained obese Zucker rats had reduced basal and insulin-stimulated
activities of ERK2 and its downstream target p90 ribosomal S6 kinase (RSK2)
. Seven weeks of training significantly increased basal and insulin-stimula
ted ERK2 and RSK2 activities, as well as insulin stimulation of MAPK kinase
activity. This effect was maintained for at least 96 h in the case of ERK2
. The training-induced increase in basal ERK2 activity was correlated with
the increase in citrate synthase activity. Therefore, 7 wk of training incr
eases basal and insulin-stimulated ERK2 activity. The increase in basal ERK
2 activity may be related to the response of muscle to training.