Chromosomal control of albumins and globulins in wheat grain assessed using different fractionation procedures

A systematic study of the water-soluble (albumin) and salt-soluble (globuli n) proteins of wheat flour, by analysis of null genetic lines using differe nt methods of protein separation, has established the location of several o f their genes on individual chromosomes. SDS-PAGE analysis of water-soluble proteins indicated the chromosomal location of polypeptides of M-chi, 64 0 00, M-chi 62 000 and M-chi, 16 000 on 4DL, 4BS and 3DS respectively. Simila rly, two globulin polypeptides of M-chi 38 000 and M-chi, 39 000 were assig ned to chromosomes 2A, 2B, 2D, 3BS, 3DS, 4DS, 5DL, 6DS, 7BS or 7DL using is oelectric focusing. In addition. several non-gluten proteins have been mapp ed on chromosome 1D, 3AL, 3BS, 3DS, 4AL, 4BS, 4DS, and 5DL by reversed-phas e HPLC (RP-HPLC). The proteins separated hy RP-HPLC were also correlated wi th proteins resolved with other methods. The polypeptides assigned to diffe rent chromosomes using SDS-PAGE and isoelectric focusing were also analysed by 2-D electrophoresis to confirm the chromosomal location of genes contro lling expression of particular polypeptides. Some other proteins that were not resolved by either method alone, were able to be mapped on chromosomes using 2-D electrophoresis. The chromosome-specific proteins are now being u sed to develop chromosome-specific markers, which may be valuable to discov er spontaneous deletions as well as natural translocations from alien speci es. (C) 2001 Academic Press.