Interleukin-1 beta regulates urokinase plasminogen activator (u-PA), u-PA receptor, soluble u-PA receptor, and plasminogen activator inhibitor-1 messenger ribonucleic acid expression in cultured human endometrial stromal cells

The interleukin-1 (IL-1) system plays an integral role in local intercellul ar interactions during implantation. In addition, the plasminogen activator system, especially urokinase plasminogen activator (u-PA), plasminogen act ivator inhibitor (PAI-1), and u-PA receptor (u-PAR), are crucial during emb ryo implantation. Decidualization and implantation are complex processes de pendent upon several proteases, including u-PA, and IL-1 is known to affect PA activity in several cell types. We investigated the role of IL-1 beta i n regulating u-PA, PAI-1, u-PAR, and soluble u-PAR messenger ribonucleic ac id (mRNA) expression in cultured human endometrial stromal cells using quan titative competitive PCR. For confirmation of the mRNA data, we measured PA I-1 and u-PAR protein by enzyme-linked immunosorbent assay. Confluent strom al cell cultures treated with progesterone and estradiol for 9 days were st imulated with IL-1 beta, and IL-1 beta plus IL-1 beta antibody for an addit ional 24 h. Total RNA was extracted, reverse transcribed, and coamplified u sing quantitative and competitive PCR with internal standards. IL-1 beta in creased PAI-1, u-PAR, and soluble u-PAR expression in a dose-dependent mann er, and this result was reversed by anti-IL-l beta antibody treatment. u-PA mRNA expression was not dependent on IL-1 beta. These results suggest that IL-1 may be important in regulating PAI-1 and u-PAR during stromal cell de cidualization before implantation.