A prevalent polymorphism in the promoter of the UCP3 gene and its relationship to body mass index and long term body weight change in the Danish population
Lt. Dalgaard et al., A prevalent polymorphism in the promoter of the UCP3 gene and its relationship to body mass index and long term body weight change in the Danish population, J CLIN END, 86(3), 2001, pp. 1398-1402
Variability of the uncoupling protein 3 (UCP3) promoter has been associated
with increased body mass index (BMI) and altered lipid profiles. Here we t
ested the hypothesis that variation of the UCP3 promoter is associated with
either juvenile or maturity-onset obesity or body weight change over a 26-
yr follow-up among Danish subjects. Mutation screening of approximately 1 k
b 5' upstream of the UCP3 gene revealed one previously described -55 C->T v
ariant. The frequency of the polymorphism was evaluated by restriction frag
ment length polymorphism analysis in four groups of subjects: 1) a group of
744 obese Danish men who at the draft board examinations had a body mass i
ndex (BMI) of at least 31 kg/m(2), 2) a randomly selected control group con
sisting of 857 draftees, 3) 258 middle-aged subjects, and 4) 409 60-yr-old
subjects. The frequency of the T allele was 26.0% (95% confidence interval,
23.8-28.2%) among the obese draftees and 26.9% (24.8-29.0%) in the control
group (P = 0.6). The variant was not associated with BMI at a young age or
with weight gain after a 26-yr follow-up. The frequency of the T allele wa
s 29.5% (25.6-33.4%) in the middle-aged group and 25.8% (22.8-28.8%) among
the 60-yr-old subjects. The polymorphism was not associated with increased
BMT or percent body fat in these 2 groups. It is concluded that this varian
t does not play a major role in the development of common obesity among Dan
ish subjects.