Acute rejection and cardiac graft vasculopathy in the absence of donor-derived ICAM-1 or P-selectin

Background: ICAM-1 and P-selectin are molecules that facilitate adhesion of circulating leukocytes to vessel walls. We have investigated the role of d onor-derived ICAM-1 and P-selectin in acute and chronic cardiac allograft r ejection. Methods: C57BL/6J (H-2(b)) mice were used as donors for heterotopic heart t ransplantation into CBA/Ca (H-2(k)) recipients. The donors were wild-type o r homozygous for gene mutations of ICAM-1 or P-selectin. We measured acute rejection in non-immunosuppressed recipients by daily palpation and sacrifi ced mice at Days 2, 4, and 6 for immunohistochemical analysis. For chronic rejection, recipients received monoclonal antibody against CD4+ T cells. We removed hearts at Days 60 to 62 for histologic assessment of vasculopathy using quantitative morphometry to measure intimal thickening. Results: Time (days) to rejection was 7.1 +/- 0.57 for wild-type (n = 10), 7.0 +/- 0.71 for ICAM-1 -/- (not significantly different, n = 7) and 6.1 +/ - 0.33 (p = 0.001) for P-selectin -/- donors. ICAM-1 deficiency was associa ted with delayed infiltrate at Day 4 compared with wild-type. In the model of chronic rejection, elastin-positive vessels showed a mean occlusion of 3 4% +/- 3% in transplanted wild-type hearts; vessels were divided into those showing 0% to 20%, 20% to 50%, and 50% to 100% occlusion. We observed no d ifference in the number of affected vessels or the amount of vascular thick ening in donors lacking ICAM-1 or P-selectin compared with wild-type contro ls. Conclusions: The absence of ICAM-1 or P-selectin in donor tissues neither l engthens the time of allograft survival nor inhibits the vascular lesions a ssociated with chronic rejection. Indeed, the absence of P-selectin may exa cerbate alloimmune injury.