M. Hannigan et al., Leukocyte-specific gene 1 protein (LSP1) is involved in chemokine KC-activated cytoskeletal reorganization in murine neutrophils in vitro, J LEUK BIOL, 69(3), 2001, pp. 497-504
Leukocyte-specific gene 1 protein (LSP1) is a cytoskeletal-associated prote
in of leukocytes that in vitro cross-links F-actin into extensively branche
d bundles of mixed polarity. In this study, we examined chemotaxis and supe
roxide production in neutrophils prepared from wild-type (WT) and Lsp1 knoc
kout mice. Compared to WT neutrophils, Ls1(-/-) neutrophils showed impairme
nt ill both migration speed and chemotaxis direction during chemokine KC-di
rected chemotaxis, When examined by confocal microscopy, chemotaxing Ls1(-/
-) neutrophils showed abnormal morphologies. They had discontinuous primary
actin-rich cortexes and large membrane protrusions. When stimulated by pho
rbol 12-myristate 13-acetate (PMA), Lsp1(-/-) peritoneal neutrophils produc
e more superoxide than WT. The? data presented suggest that LSP1 plays impo
rtant roles in the regulation of neutrophil morphology, motility, and super
oxide production.