Proton induced X-ray emission (PIXE) and atomic absorption spectroscopy (AA
S) were used for vanadium determination in animal tissues. The vanadium con
centration levels were determined in blood, kidneys and livers taken from r
ats. Two groups of the animals were treated with different diets. The diet
for the first group was supplemented with vanadium compounds while the diet
for the second one was assumed to be a "normal" diet. The second group was
treated as control. In order to achieve the best minimum detectable limit
(MDL)(1) the samples were subject to a special sample preparation procedure
. Blood and kidneys were mineralized with an APDC compound. The mineralizat
ion process was performed according to the procedure described previously.(
2) The application of PIXE3 is very useful for different types of samples.
PIXE measurements were performed with a proton beam at the Institute of Nuc
lear Physics in Cracow, Poland while the AAS measurements were done at the
Institute of Molecular Biology, Jagiellonian University, Poland. The concen
tration levels of vanadium in blood and kidneys are compared and discussed.
There were no significant statistical differences between results of vanad
ium concentration levels determined by the abovementioned techniques. The P
IXE technique had the advantage over the AAS technique of giving a broad sp
ectrum of trace elements analyzed in a single measurement. Therefore with t
he help of sample preparation procedure the application of the PIXE method
seems to be suitable for such analyzes.