Estimate of absorbed dose based on two-dimensional autoradiographic information in internal radionuclide therapy

In radiation therapies using radionuclides emitting short-range particles, such as radioimmunotherapy or boron neutron capture therapy, the biological effects are strongly affected by the heterogeneity of the absorbed dose di stribution delivered to tumor cells. The three-dimensional (3D) information of the source distribution at the cellular level is required to accurately determine the absorbed dose distribution to the individual tumor cells. Tw o-dimensional distribution of cell and nuclide with a resolution of 1 mum c an be obtained from individual tissue sections by microautoradiography. To obtain such information in 3D, an ideal approach would be to align the seri al tissue sections from a block and analyze all of them. This is straightfo rward in theory, but extremely difficult in practice. Furthermore, every se ction in the block has to be processed and analyzed, and the usage of the d ata from this laborious work is very inefficient. An approach presented her e is to estimate the absorbed dose based on individual sections without 3D reconstruction. It is realistically workable since it avoids the most diffi cult task of alignment for the serial tissue sections. In addition, the abs orbed dose can be estimated based on a limited number of noncontiguous sect ions. The validity of this approach has been tested by a Monte Carlo simula tion for two representative radionuclide configurations: (a) a uniform dist ribution of sources and (b) a cell membrane bound source distribution. With only a limited number of sampling sections, the uncertainties in the dose estimation were estimated to similar to 15% for short-range particles. (C) 2001 American Association of Physicists in Medicine. [DOI: 10.1118/1.135058 4].