Effect of PD fluid instillation on the peritonitis-induced influx and bacterial clearing capacity of peritoneal cells

Background. The commonly used peritoneal dialysis fluids contain glucose as the osmotic agent. Heat sterilization leads to the formation of glucose de gradation products which contribute, together with glucose, to the formatio n of advanced glycation end-products (AGEs). AGEs have been shown to be pre sent in the peritoneal cavity. Methods have been developed to minimize the amount of glucose degradation products in peritoneal dialysis fluids. In a rat peritoneal dialysis model, we compare the effect of a commonly used per itoneal dialysis fluid, Gambrosol(R), with a newly developed peritoneal dia lysis fluid, PD-Bio(R), on the influx and functional capacity of the perito neal cells after 2 weeks of peritoneal dialysis fluid instillation. Methods. Three groups of animals were used: rats received daily infusion wi th 15 ml of either 4% Gambrosol(R) (group 1) or 4% PD-Bio(R) (group 2), and a control group of animals did not receive fluid (group 3). After 2 weeks of PD fluid instillation, all the animals were injected. with a 0.5 ml susp ension containing 3 x 10(8) colony-forming units of Staphylococcus aureus. The in vivo bacterial clearing capacity was determined after 15 h. Results. A statistically significant higher leukocyte influx was found in t he control group compared with both PD fluid-injected groups. No statistica l differences in bacterial clearing were observed among the three groups, a lthough the number of bacteria recovered from the PD-Bio(R) group tended to be lower than that from the Gambrosol(R) group. Moreover, in both PD fluid instillation groups, the bacteria tended to be cleared more slowly compare d with the control group. The number of mesothelial cells in the PD fluid g roups was significantly greater than in the control group. Conclusion. No differences were observed in bacterial clearing capacity, le ukocyte influx and mesothelial cell number after a 2 week exposure of the p eritoneal cavity to Gambrosol(R) vs PD-Bio(R).