Interferon-gamma release in sympathetically denervated rat submaxillary lymph nodes

Objective: To examine the regulation of interferon (IFN)-gamma release by c ells derived from submaxillary lymph nodes of rats subjected to an acute or chronic superior cervical ganglionectomy (SCGx). Methods: A unilateral SCG x and a contralateral sham operation were performed. Twenty hours or 7 days later cells from submaxillary lymph nodes were incubated for 24 h without any additional treatment (experiment 1), after adding lipopolysaccharide or concanavalin A (experiment 2) or after adding norepinephrine (NE, 10(-8) M ; experiment 3). IFN-gamma concentration in the culture media was measured by ELISA. Results: Compared to controls, cells obtained from lymph nodes at a time of degeneration of sympathetic nerve terminals released more IFN-ga mma, whereas those derived from chronically SCGx lymph nodes released less IFN-gamma. Stimulation of IFN-gamma release by mitogens was detectable in t he innervated or acutely denervated lymph nodes, but not in chronically den ervated lymph nodes. When the effect of 10(-8) M NE on IFN-gamma release wa s tested, the neurotransmitter augmented cytokine release in cells prepared from chronically denervated lymph nodes only. Conclusion: The microenviron ment provided by local sympathetic nerves is essential to enable an appropr iate IFN-gamma release by submaxillary lymph node cells to occur. Copyright (C) 2001 S. Karger AG, Basel.