Adhesion of adenosine diphosphate-activated platelets to human brain microvascular endothelial cells under flow in vitro is mediated via GPIIb/IIIa

Employing video-enhanced contrast (VEC) microscopy, we examined whether TAK -029 (GPIIb/IIIa antagonist) inhibits the adhesion of activated platelets t o human brain microvascular endothelial cells (HBEC) in vitro. HBECs were c ultured on a coverglass and put in the observation chamber of VEC microscop y. Then, activated platelets by adenosine diphosphate (ADP) (2 muM) were pe rfused over HBEC at a low shear rate of 10 s(-1) for 30 min and washed out. Platelets adhered directly to HBEC. However, platelet adhesion to HBEC was suppressed when platelet rich plasma with ADP (2 muM) plus TAK-029 (GPIIb/ IIIa antagonist; 1 muM) was perfused over HBEC for 30 min and washed out. A nti-GPIb alpha. antibody (GUR20-5) did not inhibit adhesion of ADP-activate d platelets to HBEC. The above results showed adhesion of ADP-activated pla telets to HBEC under flow in vitro is mediated via GPIIb/IIIa (C) 2001 Publ ished by Elsevier Science Ireland Ltd.