TRH1 encodes a potassium transporter required for tip growth in Arabidopsis root hairs

Root hair initiation involves the formation of a bulge at the basal end of the trichoblast by localized diffuse growth. Tip growth occurs subsequently at this initiation site and is accompanied by the establishment of a polar ized cytoplasmic organization. Arabidopsis plants homozygous for a complete loss-of-function tiny root hair 1 (trh1) mutation were generated by means of the T-DNA-tagging method. Trichoblasts of trh1 plants form initiation si tes but fail to undergo tip growth. A predicted primary structure of TRH1 i ndicates that it belongs to the AtKT/AtKUP/HAK K+ transporter family. The p roposed function of TRH1 as a K+ transporter was confirmed in Rb-86 uptake experiments, which demonstrated that trh1 plants are partially impaired in K+ transport. In line with these results, TRH1 was able to complement the t rk1 potassium transporter mutant of Saccharomyces, which is defective in hi gh-affinity K+ uptake. Surprisingly, the trh1 phenotype was not restored wh en mutant seedlings were grown at high external potassium concentrations. T hese data demonstrate that TRH1 mediates K+ transport in Arabidopsis roots and is responsible for specific K+ translocation, which is essential for ro ot hair elongation.