The question whether sucrose (Suc) is present inside plastids has been long
debated. Low Suc levels were reported to be present inside isolated chloro
plasts, but these were argued to be artifacts of the isolation procedures u
sed. We have introduced Sue-metabolizing enzymes in plastids and our experi
ments suggest substantial Suc entry into plastids. The enzyme levansucrase
from Bacillus subtilis efficiently synthesizes fructan from Sue. Targeting
of this enzyme to the plastids of tobacco (Nicotiana tabacum) and potato (S
olanum tuberosum) plants leads to high-level fructan accumulation in chloro
plasts and amyloplasts, respectively. Moreover, introduction of this enzyme
in amyloplasts leads to an altered starch structure. Expression of the yea
st invertase in potato tuber amyloplasts results in an 80% reduction of tot
al Suc content, showing efficient hydrolysis of Suc by the plastidic invert
ase. These observations suggest that Suc can enter plastids efficiently and
they raise questions as to its function and metabolism in this organelle.