V. Malathi et G. Devegowda, In vitro evaluation of nonstarch polysaccharide digestibility of feed ingredients by enzymes, POULTRY SCI, 80(3), 2001, pp. 302-305
Some of the commonly used feed ingredients for poultry (corn, sorghum, fing
er millet, deoiled ricebran, soybean meal, peanut meal, sunflower meal, and
rapeseed meal) were screened for pentosans, cellulose, pectin, and total n
onstarch polysaccharides. The ingredi ent in vitro digestibilities by enzym
es were evaluated. Cereal samples screened contained mainly pentosans. Pect
in content was rich in oilseed meals. Sunflower meal, soybean meal, deoiled
rice bran, and a broiler starter diet were subjected to a two-stage in vit
ro digestion assay with three different enzyme mixtures viz., Enzyme-I (xyl
anase + cellulase from Trichoderma viridae), Enzyme-II (xylanase + cellulas
e + beta -glucanase from Huminicola insolens), and Enzyme-III (xylanase + c
ellulase + pectinase + beta -glucanase from Aspergillus aculeatus) by incub
ating 0.1 g of the sample with 3 mL of a pepsin-HCl mixture (2,000 U pepsin
/mL of 0.1N HCl) for 45 min to simulate the peptic phase of bird digestion.
A pancreatin-NaHCO3 mixture (2 mg pancreatin/mL of 1 M NaHCO3) was used fo
r 2 h at 40 C to simulate the pancreatic phase. Digestibility was assessed
by measuring the relative viscosity of the digesta supernatent and the tota
l sugars released. Enzyme-I produced the least relative viscosity and highe
st total sugars in sunflower meal, deoiled rice bran, and broiler starter d
iet, whereas Enzyme-III was very effective in soybean meal subjected to in
vitro digestion. The assay was a convenient and rapid method of screening f
or effective and stable enzymes.