Direct electrochemical response of bovine cytochrome c. 1 - Purification and purity effect on the electrochemical reaction

The direct electrochemical response of bovine heart cytochrome c was invest igated oil modified gold electrodes to establish the effect of the non-nati ve cytochrome c impurities on the voltammetric signal involving the haemic centre. Following the experimental results, it appears that the purificatio n of the protein is essential to achieve the direct electrochemistry of the metalloprotein. No remarkable differences between horse and bovine electro chemical activity were noticed using gold electrode modified with 4,4'-dipy ridyl disulfide. The calculated half-wave potential for the redox couple Fe (II)/Fe(III) front bovine cytochrome c was +262 mV vs. NHE.