The potential of oligodeoxynucleotides as mucosal and parenteral adjuvants

Synthetic oligodeoxynucleotides (ODN) containing immunostimulatory CpG moti fs (CpG ODN) are potent adjuvants in mice when delivered by parenteral (int ramuscular. subcutaneous) and mucosal (intranasal, oral and intrarectal) ro utes. We have recently shown that with mucosal delivery non-CpG ODN can als o have immunostimulatory properties which, in contrast to the Th1-bias char acteristic of CpG ODN. are predominantly Th2-like. Herein. using hepatitis B surface antigen (HBsAg) and tetanus toroid (TT) as model antigens in BALB /c mice, we have examined a number of different ODN (CpG. non-CpG. poly-T, poly-CG) to determine their effects on immune responses after mucosal (oral ) and parenteral (IM) immunizations. Our findings demonstrate that with muc osal delivery, there is a Th2-biased immunostimulatory effect that is assoc iated with non-CpG ODN, and that the presence of CpG motifs can shift this towards a Th1 response. The adjuvant effect of non-CpG ODN was much less ev ident after parenteral immunization. (C) 2001 Elsevier Science Ltd. All rig hts reserved.