Immune reactions of CD4-and CD8-positive T cell subpopulations in spleen and lymph nodes of guinea pigs after vaccination with Bacillus Calmette Guerin
T. Klunner et al., Immune reactions of CD4-and CD8-positive T cell subpopulations in spleen and lymph nodes of guinea pigs after vaccination with Bacillus Calmette Guerin, VACCINE, 19(15-16), 2001, pp. 1968-1977
Vaccination of guinea pigs with Mycobacterium bovis BCG confers partial res
istance against infection with Mycobacterium tuberculosis. Induction of imm
unity is associated with a strong T cell response. The reactions of the cyt
otoxic and helper T lymphocyte subsets after BCG vaccination were analyzed
by cytofluorometry and in functional tests. The relative number of CD8(+) T
cells in the spleen increased substantially after injection of BCG. In vit
ro restimulation after immunization induced a strong proliferative response
but no cytotoxic reactions of CD8(+) T cells against BCG-infected macropha
ges. A specific induction of IFN-gamma and RANTES mRNA was observed after v
accination particularly in CD8(+) but not in CD4(+) T cells of the lymph no
des. (C) 2001 Elsevier Science Ltd. All rights reserved.