Expression of bovine leukemia virus protein p24 in Escherichia coli and its use in the immunoblotting assay

Authors
Bicka, L Kuzmak, J Kozaczynska, B Plucienniczak, A Skorupska, A
Citation
L. Bicka et al., Expression of bovine leukemia virus protein p24 in Escherichia coli and its use in the immunoblotting assay, ACT BIOCH P, 48(1), 2001, pp. 227-232
Citations number
17
Categorie Soggetti
Biochemistry & Biophysics
Journal title
ACTA BIOCHIMICA POLONICA
ISSN journal
0001527X → ACNP
Volume
48
Issue
1
Year of publication
2001
Pages
227 - 232
Database
ISI
SICI code
0001-527X(2001)48:1<227:EOBLVP>2.0.ZU;2-H
Abstract
The gag gene encoded protein, p24 of bovine leukemia virus (BLV), was clone d and expressed as thioredoxin-6xHis-p24 protein in Escherichia coli. The b acterial cells carrying plasmid pT7THis-p24 expressed the protein of 38 kDa that was detected by immunoblotting analysis using anti-p24 monoclonal ant ibodies and sera from BLV infected cattle and sheep. The purified p24 fusio n protein was shown to be sensitive and specific for detection of BLV antib odies in the infected cattle.