Immunolocalization of glutathione-peroxidase (GSH-PO) in the steroid-producing organs and prostate

In order to confirm the relationship between glutathione-peroxidase (GSH-PO ) localization and biological significance on steroid hormone-producing org ans, immunocytochemical localization of GSH-PO in adrenal gland, testis. ov ary and prostate of the rat was investigated. GSH-PO was well stained in sp ecific cells of the tissues examined, but staining intensity was altered by the functional state of the cells. Furthermore. GSH-PO staining was modifi ed by lipoperoxidative damage in tissues or cells. Therefore, we proposed t hat the pattern of GSH-PO staining should be a more sensitive and specific indicator of oxidative damage in tissues. Thus. the staining pattern of GSH -PO is thought to be a useful marker for lipid peroxidation in the cells.