F. Lopezsanchez et al., IN-VIVO EFFECT OF DNA RELAXATION ON THE TRANSCRIPTION OF GENE RPOH INESCHERICHIA-COLI, Biochimica et biophysica acta, N. Gene structure and expression, 1353(1), 1997, pp. 79-83
The in vivo effect of Novobiocin, a gyrase inhibitor, on the transcrip
tion of gene rpoH which codes for sigma(32), the main positive regulat
or of the heat-shock response, was studied. Novobiocin induced a three
-fold increase and a slight decrease in the activity of the rpoH promo
ters P1 and P4, respectively. The Novobiocin-induced increase in the a
ctivity of promoter P1 correlates with an increase in the amount of pr
oteins sigma(32) and DnaK. These results suggest that the increase in
expression of the heat-shock proteins induced by gyrase inhibitors is
probably due to the increased activity of P1 on relaxed DNA. (C) 1997
Elsevier Science B.V.