Inhibition of a glucose-limited sequencing fed-batch culture of Salmonellaenterica serovar enteritidis by volatile fatty acids representative of thececa of broiler chickens

The effects of concentrations of volatile fatty acids on an anaerobic, gluc ose limited, and pH-controlled growing culture of Salmonella enterica serov ar Enteritidis were studied. Suddenly increasing volatile fatty acids to th e concentrations representative of the ceca of 15-day-old broiler chickens caused washout of serovar Enteritidis. In contrast, a sudden increase to th e volatile fatty acid concentrations representative of the ceca of younger broiler chickens caused a reduction in the biomass but not washout. Gradual ly increasing volatile fatty acids caused a gradual decrease in the biomass of serovar Enteritidis. We conclude that the concentrations of volatile fa tty acids present in the ceca of broilers with a mature microflora can caus e washout of serovar Enteritidis in an in vitro system mimicking cecal ecop hysiology.