Conditions for solubilization of Tamm-Horsfall protein/uromodulin in humanurine and establishment of a sensitive and accurate enzyme-linked immunosorbent assay (ELISA) method

Tamm-Horsfall protein (THP), also referred to as uromodulin, is a major gly coprotein produced by kidney cells. The level of THP in urine is an indicat or of renal function. THP levels are difficult to quantify accurately, beca use the protein forms aggregates in the pH and ion concentrations found in urine. Here, we describe conditions by which THP can be solubilized and kep t soluble during measurement using Triton X-100, EDTA, and alkaline pH. Est ablishing conditions for precise quantification of THP by enzyme-linked imm unosorbent assay (ELISA) will aid in studying the functions of THP. (C) 200 1 Academic Press.