In vitro differentiation of human marrow stromal cells into early progenitors of neural cells by conditions that increase intracellular cyclic AMP

Human marrow stromal cells (hMSCs) are multipotential stem cells that can b e differentiated into bone, cartilage, fat, and muscle. In the experiments here, we found that undifferentiated cultures of hMSCs express some markers characteristic of neural cells such as microtubule associated protein 1B ( MAP1B), neuron-specific tubulin (TuJ-1), neuron-specific enolase (NSE), and vimentin, By treating hMSCs with 0.5 mM isobutylmethylxanthine (IBMX)/1 mM dibutyryl cyclic AMP (dbcAMP) for 6 days, about 25% of the hMSCs different iated into cells with a typical neural cell morphology and with increased l evels of both NSE and vimentin. The data suggested that the hMSCs may have been differentiated into early progenitors of neural cells in vitro under c onditions that increase the intracellular level of cAMP, (C) 2001 Academic Press.