A novel homologue of the TIAP/m-survivin gene

The inhibitor of apoptosis (IAP) proteins comprise a highly conserved gene family that prevents cell death in response to a variety of stimuli. TIAP/m -survivin, a murine homologue of human Survivin, is a member of the LAP fam ily. TIAP/m-survivin has one baculovirus LAP repeat (BIR) and lacks a C-ter minal RING; finger motif. Here we identified the genomic DNA region (TIAP-2 ) that is homologous to the TIAP/m-survivin gene by a low stringency genomi c DNA hybridization. The region is on the chromomsome 9 which is distinct f rom that (chromosome 11) of the TIAP/m-survivin gene, and contains DNA sequ ence similar to a part of the BIR and the 3' side of the TIAP/m-survivin ge ne and the sequence homology between them is 92%. Expression of TIAP-2 mRNA was detected in various murine tissues by RT-PCR. Although expression of T IAP/m-survivin mRNA is upregulated in synchronized cells at S to G2/M phase of the cell cycle, expression of TIAP-2 mRNA was constant in the cell cycl e, suggesting the different role of TIAP-2 from that of TIAP/m-survivin. (C ) 2001 Academic Press.