Ob. Belyaeva et al., Participation of free radicals in photoreduction of protochlorophyllide tochlorophyllide in an artificial pigment-protein complex, BIOCHEM-MOS, 66(2), 2001, pp. 173-177
The primary stages of protochlorophyllide phototransformation in an artific
ially formed complex containing heterologously expressed photoenzyme protoc
hlorophyllide-oxidoreductase (POR), protochlorophyllide, and NADPH were inv
estigated by optical and ESR spectroscopy. An ESR signal (g = 2.002; H = 1
mT) appeared after illumination of the complex with intense white light at
77 K. The ESR signal appeared with simultaneous quenching of the initial pr
otochlorophyllide fluorescence, this being due to the formation of a primar
y non-fluorescent intermediate. The ESR signal disappeared on raising the t
emperature to 253 K, and a new fluorescence maximum at 695 nm belonging to
chlorophyllide simultaneously appeared. The data show that the mechanism of
protochlorophyllide photoreduction in the complex is practically identical
to the in vivo mechanism: this includes the formation of a short-lived non
-fluorescent free radical that is transformed into chlorophyllide in a dark
reaction.