Heme oxygenase-1 induction by nitric oxide in RAW 264.7 macrophages is upregulated by a cyclo-oxygenase-2 inhibitor

Authors
Alcaraz, MJ Habib, A Creminon, C Vincente, AM Lebret, M Levy-Toledano, S Maclouf, J
Citation
Mj. Alcaraz et al., Heme oxygenase-1 induction by nitric oxide in RAW 264.7 macrophages is upregulated by a cyclo-oxygenase-2 inhibitor, BBA-GEN SUB, 1526(1), 2001, pp. 13-16
Citations number
20
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
ISSN journal
03044165 → ACNP
Volume
1526
Issue
1
Year of publication
2001
Pages
13 - 16
Database
ISI
SICI code
0304-4165(20010403)1526:1<13:HOIBNO>2.0.ZU;2-U
Abstract
Unstimulated RAW 264.7 macrophages express negligible heme oxygenase-1 (HO- 1) protein but incubation with the nitric oxide (NO) donor spermine nonoate (SPNO) induced HO-1 and weakly cyclo-oxygenase-2 (COX-2) protein. This eff ect was potentiated by coincubation with the COX-2 selective inhibitor. SC5 8125. Cells incubated with SPNO showed a strong increase in HO-1 mRNA level s after 4 h with a significant potentiation in the presence of SC58125, whi ch did not modify HO-1 mRNA stability. The induction of HO-1 by NO and its potentiation by anti-inflammatory agents may play a role in inflammatory an d immune responses. (C) 2001 Elsevier Science B.V. All rights reserved.