Comparison of chromosome damage induced by three zinc compounds using human leukocyte culture

The degree of chromosome damage induced by three compounds of zinc (zinc ch loride, zinc sulfate, zinc acetate) was compared in human leucocytes in vit ro. Three concentrations of each salt, 3.0 x 10(-5)M, 3.0 x 10(-4)M, and 1. 5 x 10(-3)M, were added to leukocyte cultures. The cells were harvested aft er 48 and 72 h and chromosome spreads were prepared following a colchicine- hypotonic-fixation-air drying-Giemsa staining schedule. The end point scree ned was chromosome aberrations. All three salts were lethal at the highest concentration. The degree of chromosome damage was directly proportional to the concentrations used for zinc sulfate and zinc acetate but not for zinc chloride.