Chemo-enzymatic synthesis of 3-(2-naphthyl)-L-alanine by an aminotransferase from the extreme thermophile, Thermococcus profundus

Authors
Hanzawa, S Oe, S Tokuhisa, K Kawano, K Kobayashi, T Kudo, T Kakidani, H
Citation
S. Hanzawa et al., Chemo-enzymatic synthesis of 3-(2-naphthyl)-L-alanine by an aminotransferase from the extreme thermophile, Thermococcus profundus, BIOTECH LET, 23(8), 2001, pp. 589-591
Citations number
7
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
23
Issue
8
Year of publication
2001
Pages
589 - 591
Database
ISI
SICI code
0141-5492(200104)23:8<589:CSO3BA>2.0.ZU;2-O
Abstract
Hyper-thermostable aminotransferase from Thermococcus profundus (MsAT) was used to synthesize 3-(2-naphthyl)-L-alanine (Nal) by transamination between its corresponding alpha -keto acid, 3-(2-naphthyl)pyruvate (NPA) and L-glu tamate (Glu) at 70 degreesC. Equilibrium of this reaction was shifted towar d Nal production due to its low solubility, giving rise to Nal precipitate. Optically pure Nal (> 99% ee) was synthesized with 93% (mol mol(-1)) yield from 180 mM NPA and 360 mM Glu.