On-line electrocatalyzed luminol chemiluminescence determination of D-glucose and dissolved oxygen in simulated mammalian cell bioreactor perfusion fluid using solid phase reagent modules

On-line instrumentation and methods for the chemiluminescence based real-ti me monitoring of D-glucose and O-2 levels in mammalian cell bioreactor perf usion fluid are described. The unit processes required for the analysis inc lude: pH adjustment using solid phase flow-through modules, immobilized enz yme catalyzed oxidation of glucose by molecular oxygen to produce hydrogen peroxide, controlled release of luminol using a solid phase flow-through mo dule, electrocatalyzed luminescence using gold electrodes, and photodetecti on of chemiluminescent emissions. Calibration curves for D-glucose and diss olved O-2 in simulated bioreactor perfusion fluid have been generated using fully integrated reagentless test systems from 0-800 mg l(-1) and 0-10 mg l(-1), respectively.