High-level expression of bovine rotavirus VP4 protein in Escherichia coli using a hepatitis B core antigen vector

Authors
Ibrahim, N McCrae, MA
Citation
N. Ibrahim et Ma. Mccrae, High-level expression of bovine rotavirus VP4 protein in Escherichia coli using a hepatitis B core antigen vector, BIOTECH LET, 23(8), 2001, pp. 643-646
Citations number
17
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
23
Issue
8
Year of publication
2001
Pages
643 - 646
Database
ISI
SICI code
0141-5492(200104)23:8<643:HEOBRV>2.0.ZU;2-C
Abstract
Six regions of the VP4 protein of bovine rotavirus strain UKtc were express ed using hepatitis B core antigen (HBcAg) as a carrier. Following induction by IPTG, the six fusion proteins, AHBcAg through FHBcAg, were expressed in Escherichia coli to a level of 20-37% of total cellular protein. Soluble f usion proteins in a particulate form were partially purified with yields ra nging from 0.5-6.4 mg l(-1) of culture.