Enhanced production of penicillin G acylase from a recombinant Escherichiacoli

Authors
Vohra, PK Sharma, R Kashyap, DR Tewari, R
Citation
Pk. Vohra et al., Enhanced production of penicillin G acylase from a recombinant Escherichiacoli, BIOTECH LET, 23(7), 2001, pp. 531-535
Citations number
12
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
23
Issue
7
Year of publication
2001
Pages
531 - 535
Database
ISI
SICI code
0141-5492(200104)23:7<531:EPOPGA>2.0.ZU;2-L
Abstract
Penicillin G acylase (pac) gene was cloned into a stable asd(+) vector (pYA 292) and expressed in Escherichia coli. This recombinant strain produced 10 00 units penicillin G acylase g(-)1 cell dry wt, which is 23-fold more than that produced by parental Escherichia coli ATCC11105. This enzyme was puri fied to 16 units mg(-1) protein by a novel two-step process.