Properties of recombinant Rhizomucor miehei lipase with amino acid substitutions of Phe94 in the substrate binding domain

Authors
Oh, SW Gaskin, DJH Young Kwon, D Vulfson, EN
Citation
Sw. Oh et al., Properties of recombinant Rhizomucor miehei lipase with amino acid substitutions of Phe94 in the substrate binding domain, BIOTECH LET, 23(7), 2001, pp. 563-568
Citations number
16
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
23
Issue
7
Year of publication
2001
Pages
563 - 568
Database
ISI
SICI code
0141-5492(200104)23:7<563:PORRML>2.0.ZU;2-S
Abstract
The chain length specificity of Rhizomucor miehei lipase was altered by sub stituting Phe94 in the protein groove which is responsible for accommodatin g the acyl chain of the substrate. Three recombinant enzymes, Phe94Arg, Phe 94Glu and Phe94Gln, were expressed in Pichia pastoris, purified and their a bility to hydrolyse p-nitrophenyl esters and triacylglycerols of different chain length was studied.