Enzymatic glycosylation using 6-O-acylated sugar donors and acceptors: beta-N-acetylhexosaminidase-catalysed synthesis of 6-O,N,N '-triacetylchitobiose and 6 '-O,N,N '-triacetylchitobiose

Authors
Husakova, L Riva, S Casali, M Nicotra, S Kuzma, M Hunkova, Z Kren, V
Citation
L. Husakova et al., Enzymatic glycosylation using 6-O-acylated sugar donors and acceptors: beta-N-acetylhexosaminidase-catalysed synthesis of 6-O,N,N '-triacetylchitobiose and 6 '-O,N,N '-triacetylchitobiose, CARBOHY RES, 331(2), 2001, pp. 143-148
Citations number
10
Categorie Soggetti
Agricultural Chemistry","Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
CARBOHYDRATE RESEARCH
ISSN journal
00086215 → ACNP
Volume
331
Issue
2
Year of publication
2001
Pages
143 - 148
Database
ISI
SICI code
0008-6215(20010322)331:2<143:EGU6SD>2.0.ZU;2-R
Abstract
p-Nitrophenyl 6-O-acetyl-2-acetamido-2-deoxy-beta -D-glucopyranoside (5a) w as used as the glycosyl donor in a beta -N-acetylhexosaminidase-catalysed ( from Penicillium brasilianum) glycosylation of GlcNAc yielding 6'-O,N,N'-tr iacetylchitobiose (6), while 6-O-acetyl-2-acetamido-2-deoxy-beta -D-glucopy ranose (3a) served as a selectively protected acceptor in a transglycosylat ion reaction catalysed by the same enzyme to yield 6-O,N,N'-triacetylchitob iose (4). (C) 2001 Elsevier Science Ltd. All rights reserved.