The exopolysaccharide of Streptococcus thermophilus S3, produced in skimmed
milk, is composed of D-galactose and L-rhamnose in a molar ratio of 2:1. T
he polysaccharide contains 0.4 equiv of O-acetyl groups per repeating unit.
Linkage analysis and 1D/2D NMR (H-1 and C-13) studies on native and O-deac
etylated EPS together with nanoES-CID tandem mass spectrometry studies on o
ligosaccharides generated by a periodate oxidation protocol, show the polys
accharide to have the following structure:
[GRAPHICS]
(C) 2001 Elsevier Science Ltd. All rights reserved.