Sweeping of neutral analytes via complexation with borate in capillary zone electrophoresis

Citation
Jp. Quirino et S. Terabe, Sweeping of neutral analytes via complexation with borate in capillary zone electrophoresis, CHROMATOGR, 53(5-6), 2001, pp. 285-289
Citations number
17
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
CHROMATOGRAPHIA
ISSN journal
00095893 → ACNP
Volume
53
Issue
5-6
Year of publication
2001
Pages
285 - 289
Database
ISI
SICI code
0009-5893(200103)53:5-6<285:SONAVC>2.0.ZU;2-R
Abstract
The sweeping concept is extended to capillary zone electrophoresis (CZE) se paration of neutral solutes involving complexation with berate. Analogous i o the pseudostationary phase in electrokinetic chromatography (EKC), the co mplexing agent (borate) serves as carrier for sweeping and separation in CZ E. Therefore, similar to the retention factor in EKC, the focusing effect i n the present system is directly related io the association constant betwee n the analyte and complexing agent. Theoretical and some preliminary experi mental studies generally suggest that the electrophoretic mobility of the c omplex and the concentration of the complexing agent affect the resulting l ength of narrowed zones. Moreover, sweeping using berate is affected by pH since borate complexation is pH dependent. From around 10 to 40-fold improv ement in peak heights has been observed experimentally for some neutral les t analytes (monosaccharides, catechols, and nucleosides)