HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY SEPARATION ACID LIGHT-SCATTERING DETECTION OF PHOSPHOLIPIDS FROM COOKED BEEF

A sensitive high-performance liquid chromatography (HPLC) method for t he separation and quantitative analysis of major phospholipids (PLs) i n biological systems is described. PLs were purified by solid-phase ex traction with an amino (NH2) phase. Separation of PLs was carried out on an HPLC silica gel column, with a mobile phase consisting of chloro form, methanol and ammonium hydroxide, and detection was performed wit h a light-scattering evaporative detector. HPLC analysis of PLs extrac ted from ground beef cooked under different conditions and capillary g as chromatography of the fatty acid methyl esters showed that cooking treatments did not have a significant effect on the PL composition and fatty acid contents of the single PLs in ground beef.