Multi-modal combination gene therapy for malignant glioma using replication-defective HSV vectors

Herpes simplex virus (HSV) may be modified to produce a nonpathogenic vecto r that is capable of delivering multiple transgenes simultaneously to cells , both safely and efficiently. We have exploited this property to develop v iruses that target glioblastoma, a malignancy that is currently associated with a poor prognosis. Using rationally selected combinations of therapeuti c transgenes coupled with gamma-knife radiotherapy, the ablation of experim ental tumours in animal models has been demonstrated. Combination gene ther apy using replication-defective HSV vectors represents a promising and exci ting approach to tackling malignancy in the CNS.