Plo1-associated casein kinase activity peaked during mitosis before septati
on. Phosphatase treatment abolished this activity, Mitotic Plo1 activation
had a requirement for prior activation of M-phase promoting factor (MPF), s
uggesting that Plo1 does not act as a mitotic trigger kinase to initiate MP
F activation during mitotic commitment, A link between Plo1 and the septum
initiating network (SIN) has been suggested by the inability of plo1 Delta
cells to septate and the prolific septation following plo1(+) overexpressio
n, Interphase activation of Spg1, the G protein that modulates SIN activity
, induced septation but did not stimulate Plo1-associated kinase activity.
Conversely, SIN inactivation did not affect the mitotic stimulation of Plo1
-associated kinase activity, plo1,ts4 cells formed a misshapen actin ring,
but rarely septated at 36 degreesC, Forced activation of Spg1 enabled plo1.
ts4 mutant cells, but not cells with defects in the SIN component Sid2, to
convert the actin ring to a septum, The ability of plo1(+) overexpression t
o induce septation was severely compromised by SIN inactivation, We propose
that Plo1 acts before the SIN to control septation.