Chlorophyll derived from Chlorella inhibits dioxin absorption from the gastrointestinal tract and accelerates dioxin excretion in rats

We investigated the effects of chlorophyll derived from Chlorella on gastro intestinal absorption of seven types of polychlorinated dibunzo-p-dioxin (P CDD) and 10 types of polychlorinated dibenzofuran (PCDF) in Wistar rats. Tw enty-eight rats were randomly distributed into seven groups (n = 4). After overnight food deprivation, rats were given 4 g of the basal diet or 4 g of the chlorophyll diet containing 0.01-0.5% chlorophyll one time on day 1; e ach diet also contained 0.2 mt PCDD and PCDF standard solutions. The amount s of fecal excretion of PCDD and PCDF congeners from days 1 to 5 in the gro up fed 0.01% chlorophyll were 64.8% for 1,2,3,7,8-pentaCDD, 78.6% for 1,2,3 ,4,7,8-hexaCDD, 73.5% for 1,2,3,Ii,7,8-hexaCDD, 58.5% for 1,2,3,7,8,9-hexaC DD, 33.3% for 1,2,3,4,Ii,7,8-heptaCDD, 85.7% for 1,2,3,7,8-pentaCDF, 77.3% for 2,3,4,7,8-pentaCDF, 88.6% For 1,2,3,4,7,8-hexaCDF, 78.0% for 1,2,3,li,7 ,8-hexaCDF, 62.5% for 1,2,3,7,8,9-hexaCDF, 84.1% for 2,3,4,6,7,8-hexaCDF, 4 1.7% for 1,2,3,4,6,7,8-heptaCDF, and 40.0% for 1,2,3,4,6,7,8-heptaCDF great er (p < 0.01) than those of the control group, respectively. The fecal excr etion of PCDD and PCDF congeners was remarkably increased along with the in creasing dietary chlorophyll. The amounts of PCDD and PCDF congeners in rat s on day 5 administered dioxin mixtures were lower in the 0.01% chlorophyll group than in the control group, ranging from 3.5 to 50.0% for PCDD congen ers and from 3.7 to 41.7% lower for PCDF congeners, except for 2,3,7,8-tetr achlorodibenzofuran, The amount of PCDD and PCDF congeners in rats was rema rkably decreased along with the increasing dietary chlorophyll.