Alternative activation of extracellular signal-regulated protein kinases in curcumin and arsenite-induced HSP70 gene expression in human colorectal carcinoma cells

We have investigated the regulation mechanism of chemical stress-induced HS P70 gene expression in human colorectal carcinoma cells (COLO205 and HT29). Our data show that chemical treatments including sodium arsenite and curcu min, induced significant synthesis of HSP70 and its mRNA, The induced HSP;I O gene expression appears to be increased at the transcriptional level. The increase in HSP70 gene expression by both chemicals is associated with an increase in HSF binding to HSE and induction of HSF1 di- or trimerization, Phosphorylation and activation of extracellular signal-regulated proteins ( ERK1/2) were detected in sodium arsenite-treated COL0205 and HT29 cells, an d the free radical scavenger N-acetyl-Lcysteine (NAC) was able to inhibit t his ERK1/2 activation and HSP70 gene expression. MAPK blockade by the speci fic MEK1 inhibitor (PD98059) decreased the ability of sodium arsenite to in crease HSP70 gene expression in a dose-dependent manner along with dephosph orylation of ERK1/2 proteins. in contrast to arsenite treatment, activation of ERK1/2 was not detected in curcumin-treated colorectal carcinoma cells, and NBC and PD98059 did not show any inhibitory effect on HSP70 gene expre ssion induced hy curcumin, Overexpression of a dominant negative mutant of mitogen-activated protein kinase kinase kinase 1 (MEKK1-DN) prevents arseni te-induced ERK1/2 phosphorylation and HSP70 protein synthesis. These result s indicated that the ERK signaling pathway can participate in HSP70 gene ex pression induced by the prooxidant sodium arsenite, but not by the antioxid ant curcumin.