The mode of inheritance of chloroplast DNA has been determined in Encephala
rtos by employing a restriction fragment length polymorphism analysis of ch
loroplast DNA. Artificial F-1 hybrids were produced between a female specim
en of E. natalensis and a male specimen of E. woodii. The hybridization pat
terns of all hybrids correspond, in all cases, with that of E. natalensis,
and are different from that of E. woodii, thus indicating the maternal inhe
ritance of cpDNA in cycads.