Mould strains were isolated from Olive Mill Waste (OMW) waters, characteriz
ed and screened for their activities on polyphenols hydrolysis by reculturi
ng on the olive mill waste waters itself. The selected strains were then us
ed for polyphenols removal in pure culture assays in flasks. Prior to the i
noculation, OMW water was diluted to prepare three initial concentrations o
f polyphenols (2.2 g/L; 4.5g/L; 14.5g/L). Chemical characteristics includin
g polyphenols and Chemical Oxygen Demand (COD) were followed up in these as
says. Results showed that the polyphenols removal by some strains was estim
ated to around 95.14% and the COD was also decreased by 43%. Olive mill was
te waters are normally colored in dark because of the polyphenols oxidation
. The microbial strains by hydrolysing the polyphenols had decolored comple
tely these effluents and a clear liquid was obtained after 12-15 days of tr
eatment under aerobic shaking in laboratory. Polyphenols may have some inhi
bitory effect which would cause problems to the biological treatment of the
se effluents. Their removal would facilitate the treatment of the waste.