Primary structure deduction and molecular modelling from a cDNA of a cellobiohydrolase-like protein from the white-rot fungus Coriolus versicolor

Molecular cloning and cDNA sequencing analysis were used to elucidate the p rimary structure of a cellulase-like structure from the white-rot fungus Co riolus versicolor. The cDNA of interest was isolated from a cDNA library ob tained from C. versicolor mycelia grown on cellulase inducer medium. A patt ern search showed that this cellulase belongs to the glycosyl hydrolases fa mily 6. From the deduced amino acid sequence, models of the binding and cat alytic domains were built by homology modelling. The constructed models pre sent a typical cellulose-binding domain at the N-terminal region, a rich Pr o, Ser. Thr linker peptide, and a catalytic domain at the C-terminus region . (C) 2001 Elsevier Science B.V. All rights reserved.