Glutathione S-transferases in small intestinal mucosa of patients with coeliac disease

Patients with villous atrophy due to coeliac disease have an increased risk of developing small intestinal malignancies. Intestinal glutathione (GSH) and glutathione S-transferases (GST) are involved in the protection against carcinogenesis. The aim of this study was to evaluate GSH content and GST enzyme activity in small intestinal mucosa of untreated coeliacs compared t o controls. We evaluated GSH content and GST enzyme activity, including the levels of GST classes alpha, mu, pi and theta, in small intestinal biopsie s of untreated coeliacs (flat mucosa, Marsh IIIC, n=12) compared to normal subjects (n=23), Next, we evaluated GSH and GST's in coeliacs in remission (Marsh 0-I, n=11), coeliacs with persisting villous atrophy while on a glut en-free diet (partial villous atrophy, Marsh IIIA (n=5); subtotal villous a trophy, Marsh IIIB (n=6)) and patients with infiltrative/crypt-hyperplastic Marsh II lesions (n=4). Total GST enzyme activity and content of GST alpha are markedly suppressed in Marsh IIIC lesions compared to controls (resp, 220 +/- 79 vs. 464 +/- 189 nmol/mg protein min (P<0.001) and 2.79<plus/minu s>2.36 vs. 6.47 +/-2.29 mug/mg protein (P<0.001)), In coeliacs in remission these levels normalized. Total GST enzyme activity and GST<alpha> levels a re proportionately lowered according to the degree of mucosal pathology in Marsh II, IIIA and IIIB, (Spearman's sigma correlation coefficient for tota l GST, -0.596, P<0.001; GST<alpha>, -0.620, P<0.001), GST<mu>, pi and theta and GSH levels are not significantly different in the selected study group s of mucosal pathology compared to controls. Total CST enzyme activity and content of GST alpha in small intestinal mucosa are significantly lower in untreated coeliac disease compared to controls, In Marsh II, IIIA and IIIB, CST enzyme activity and GSTa content are proportionally lower according to the degree of mucosal pathology. Normal values are seen in coeliacs in rem ission. This correlation between coeliac disease and a suppressed GSH/GST d etoxification system may explain in part the carcinogenic risk in untreated coeliac disease.