Intracameral and lenticular penetration of locally applied stable isotope-labeled vitamin E

Purpose: To confirm the aqueous humor and lens dynamics of 1% deuterium-lab eled alpha -tocopherol acetate (D-3-VEA) solution. Methods: The concentrations of D-3-VEA and D-3-alpha -tocopherol (D-3-VE), derived from D-3-VEA, in the aqueous humor and lens were measured after con tinuous instillation of 1% D-3-VEA into the cul-de-sac of rat eyes for 1 or 3 weeks. D-3-VEA and D-3-VE concentrations were determined by gas chromato graphy/mass selective detector. Results: In the aqueous humor, the concentrations of D-3-VEA and D-3-VE wer e, respectively, 93.1 and 9.4 ng/mL after 1 week, and, respectively, 498.9 and 21.5 ng/mL after 3 weeks. In the lens, they were, respectively, 15.0 an d 9.8 ng/g after 3 week, and 6.1 and 4.8 ng/g after 3 weeks. Conclusion: The penetration levels of alpha -tocopherol acetate by eyedrop application were confirmed in the aqueous humor and lens. (C) 2001 Japanese Ophthalmological Society.